Ribonucleic acids from animal cells.

نویسنده

  • J E Darnell
چکیده

INTRODUCTION ................................................................ 262 TECHNICAL ASPECTS OF RNA BIOCHEMISTRYC. .. 263 Size ...263 Compositio.... . 263 Methylation . .263 RNA-DNA Hybridization ...264 Cell Fractionation ......................................................... 264 PATTERNS OF SYNTHESIS OF ANIMAL CELL R.. ........ .. 264 "Rapidly Labeled RNA" and Kinetic Analysis Experimes .... 264 BIOSYNTHESIS OF RlBOSOMES IN ANIMAL CELLS . .. 267 Ribosomal PrecursorRNA..... 267 Formation and Distribution of Ribosomal Particles .. 272 Ribosomal Proteins and Nucleolar Ribosomal Precursors 274 Association of Ribosomal Protein withrRNA.. 276 Place of Small RNA Molecules in RibosomalMaturatiot.. 276 Control of Ribosome Formation.277 PRECURSORS TO TRNA MOLECULES ...279 DNA-LIKE RNA SPECIES...................................................... 280 Definition and Distribution of DNA-like RNA 280 HnRNA: Properties and Problems.283 Use ofRNA-DNA Hybridizationz in the Study ofDNA-like RNA Species.284 CONCLUSIONS ............................................. 286 LITERATURE CITED ........................................................... 287

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues.

The methods used at present for the determination of the higher nucleic acids in organs are based on certain color reactions of their carbohydrate components. The quantitative evaluation of these color tests gives satisfactory results with the free carbohydrates and with the purine nucleosides and nucleotides. The application of the color tests to the higher nucleic acids, however, encounters s...

متن کامل

Improved methods for purification and assay of eukaryotic messenger ribonucleic acids and ribosomes. Quantitative analysis of their interaction in a fractionated reticulocyte cell-free system.

The polyadenylic acid-containing messenger ribonucleic acids of eukaryotic cells are rapidly isolated and deproteinized in a simple and gentle one-step procedure. The polyribosome fraction, dissolved in 0.5 M NaCl/0.5 percent sodium dodecyl sulfate, is passed through an oligo(dT)-cellulose column which is then washed with the solvent until proteins and contaminating ribonucleic acids are fully ...

متن کامل

Characteristics of the syrian hamster ribonucleic acid present in cells transformed by polyoma, simian virus 40, or adenovirus 12.

Ribonucleic acids of known specific activities were separately prepared from hamster cells transformed by polyoma, simian virus 40 (SV40), and adenovirus 12. When increasing amounts of these labeled ribonucleic acids were added to nitrocellulose filters containing immobilized (14)C-hamster deoxyribonucleic acid (DNA), about 5% of the DNA sites were saturated in each case. An additive saturation...

متن کامل

Ribonucleic Acids in Virus-infected and Uninfected Krebs Ii Ascites Tumor Cells.

Two lines of evidence support the view that the replication of ribonucleic acid-containing animal viruses proceeds via RNAdirected synthesis of RNA. It has been shown that RNA viruses continue to replicate under conditions in which synthesis of deoxyribonucleic acid and the DNB-dependent synthesis of RNA are artificially suppressed (l-3). Furthermore, in certain mammalian cell lines there is ev...

متن کامل

The preparation and characterization of ribonucleic acids from yeast.

Considerable variation in composition occurs among samples of ribonucleic acids from yeast. These variations are due in part to the procedures that are employed for isolation and subsequent purification. A recent study by Loring, Fairley, and Seagran (1) notes that variations may be due to (a) the occurrence of different ribonucleic acids in the particulate components of cells, (b) partial enzy...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Bacteriological reviews

دوره 32 3  شماره 

صفحات  -

تاریخ انتشار 1968